Carbohydrate Lab Report Essay

Qualitative identification of a substance is of significant importance in chemistry. Physical constants much(prenominal) as melting points have traditionally been used by organic chemistry for identification of unexplored compounds. As for inorganic substances, the precipitation of a solid, firmness of purposes of a flame test, or the formation of a sour substance could all be keys to recogniseing a sample. Chromatography and spectra are amongst the newer techniques practiced today. A problem that arises in biochemistry when attempting to identify a particular compound is that the tests that are utilized to identify these compounds rely on the reaction of a functional group and therefore will yield positive reactions with more than just one compound. This substance that more than one test has to be performed in order to deduce which compound is present by process of elimination. Carbohydrate chemistry allows us a great opportunity to better understand qualitative testing. The t ests run within this experiment are usually carried out to identify simple pelfs. It is possible that by running these tests with a variety of different carbohydrates we determine the identity of an unknown sample. examinationsBenedicts visitation rills for Reducing Sugar (+), Nonsimplification Sugar ()Positive result Red precipitate Negative result No precipitate Bials TestTests for Pentoses (+), Other ()Positive result channelize in coloration to swampy dark blue Negative result Not cloudy dark blue Barfoerds TestTests for Monosaccharides (+), Disaccharides ()Positive result Red precipitate Negative result No precipitate Seliwanoffs TestTests for Fructose (+), Other ()Positive result Change in color to orange Negative result Not orange Glucose Oxidase TestTests for Glucose (+), Other ()Positive result Changes color Negative result the Great Compromiser same color Starch Iodine TestTests for Starch (+), Sucrose ()Positive result Change in color to blue Negative result Not bl ueDiscussionWe determined our carbohydrate to be ribose considering it matched up perfectly with all the test results we had ran. Ribose was the only carbohydrate of the ten that had similar reactions to the tests performed. The trey tests that ribose was subject to were Benedicts test, Bials test, and Barfoerds test, all of which ribose tested positive for. Since the unknown besides tested positive to these three tests and negative to all other tests that we ran, this means the unknown is a reducing sugar, a pentose, and a monosaccharide. We did have a bit of trouble trying to decide whether the unknown was arabinose or ribose because they both tested positive to the aforementioned tests. However, arabinose had a greenish tint and our unknown matched in color more closely to riboses dark blue.Read moreIodine Test for Starch research lab Report